SRP3177

TNF-α human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

• 동의어(들): Cachectin, Cytotoxin, Differentiation-inducing factor (DIF), Necrosin, Tumor Necrosis Factor
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥95% (SDS-PAGE)
• 양식: lyophilized
• 효능: ≤0.10 ng/mL ED₅₀
• 분자량: 17.4 kDa
• 포장: pkg of 50 μg
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1.00 EU/μg endotoxin
• UniProt 수납 번호: P01375
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... TNF(7124)

설명

일반 설명

TNF-α (tumor necrosis factor α) is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. The gene is mapped to human chromosome 6p21.3. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

애플리케이션

TNF-α (tumor necrosis factor α) human has been used as a positive control for induction of proinflammatory genes in human intestinal and hepatic cell lines.

생화학적/생리학적 작용

TNF-α (tumor necrosis factor α) is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. However, it can be a tumor promoter as well as a tumor suppressor. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, apoptosis and diabetes. It is also associated with mood and anxiety disorders.

서열

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

물리적 형태

Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 0.5mM DTT.

재구성

It is recommended to centrifuge the vial beforereconstitution. A stock solution may be prepared byreconstitution in sterile water to a concentration of0.1 mg/mL. The stock solution may be diluted furtherusing culture media.

저장 및 안정성

Store the product at −20 °C. After reconstitution, theproduct can be stored at 2-8 °C for up to 1 month.For extended storage, freeze in working aliquots.Repeated freezing and thawing are not recommended.Do not store in a frost-free freezer.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable