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Merck
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Key Documents

11088939001

Roche

Tumor Necrosis Factor-α, human (hTNF-α)

recombinant (yeast)

동의어(들):

hTNF, human necrosis factor

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About This Item

UNSPSC 코드:
12352207

생물학적 소스

human

Quality Level

재조합

expressed in yeast

분석

≥95% (SDS-PAGE)

형태

solution

효능

≤0.01 ng/mL EC50

특이 활성도

>1*10^8 U/mg

분자량

17,000 Da

포장

pkg of 1,000,000 U (10 μg, 1ml)

제조업체/상표

Roche

저장 조건

avoid repeated freeze/thaw cycles

불순물

<0.1 EU/μgtested (LAL test)

UniProt 수납 번호

저장 온도

−20°C

유전자 정보

human ... TNF(7124)

일반 설명

>1 x 108 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.

특이성

Human hTNF-α is effective on human and mouse cells.
Species specificity: human, mouse

애플리케이션

Tumor Necrosis Factor-α (hTNF-α)
  • Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
  • Has activating and growth stimulating activities on a variety of normal cells.
  • Has antiviral activity on many cell types in vitro.
  • Can cause tumor necrosis of certain tumors in vivo.

생화학적/생리학적 작용

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

품질

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

서열

Chain Length 157 AA
One polypeptide chain (157 amino acids), identical to natural hTNF-α.

단위 정의

EC50 definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).

물리적 형태

Solution, filtered through 0.2 μm pore size membrane.

제조 메모

Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

기타 정보

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


시험 성적서(COA)

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문서 라이브러리 방문

P De Cesaris et al.
The Journal of biological chemistry, 274(41), 28978-28982 (1999-10-03)
Tumor necrosis factor alpha (TNF-alpha) is a cytokine implicated in the pathogenesis of numerous chronic and acute inflammatory conditions. We have previously shown that mouse Sertoli cells respond to TNF-alpha by increasing interleukin-6 production and intercellular adhesion molecule-1 (ICAM-1) expression
Rajitha Indukuri et al.
Frontiers in endocrinology, 12, 650625-650625 (2021-04-17)
Inflammation is a primary component of both initiation and promotion of colorectal cancer (CRC). Cytokines secreted by macrophages, including tumor necrosis factor alpha (TNFα), activates the pro-survival transcription factor complex NFκB. The precise mechanism of NFκB in CRC is not

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