SRP0722

KRAS (Mature Form), Active

PRECISIO^® Kinase, recombinant

• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 재조합: expressed in E. coli
• 분석: 70-95% (SDS-PAGE)
• 양식: frozen liquid
• 특이 활성도: 2.0-3.5 nmol/min·mg (GTPase-Glo)
• 분자량: ~23 kDa
• 농도: 0.1 μg/μL
• 수납 번호(accession number): NM_033360
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... KRAS(3845)

설명

애플리케이션

Useful for enzyme assay and Western blot.

생화학적/생리학적 작용

Recombinant human KRAS (2-186) was expressed in E.coli cells using an N-terminal His tag. The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking ⁽¹⁾. There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types ⁽²⁾. Other members of the RAS superfamily may also contribute to cancer.

재구성

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

저장 및 안정성

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

법적 정보

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H360D
• 예방조치 성명서: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable