SRP0320

JMJD2b active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

• 동의어(들): KDM4B, lysine (K)-specific demethylase 4B
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected Sf9 cells
• 분석: ≥70% (SDS-PAGE)
• 형태: aqueous solution
• 분자량: 83 kDa
• 포장: pkg of 100 μg
• NCBI 수납 번호: NM_015015
• UniProt 수납 번호: O94953
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... KDM4B(23030)

설명

일반 설명

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), amino acids 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.

특이성

0.32 pmole/min/μg Assay conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM a-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin conjugated secondary antibody followed by Alpha Screening detection.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

물리적 형태

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol.

저장 및 안정성

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable