SRP0212

PTP1B (1-321)/PTPN1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 동의어(들): PTPN1, Protein tyrosine phosphatase, non-receptor type 1
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥90% (SDS-PAGE)
• 형태: aqueous solution
• 분자량: 63.3 kDa
• 포장: pkg of 20 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: 0.84 mg/mL
• NCBI 수납 번호: M31724
• UniProt 수납 번호: P18031
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PTPN1(5770)

설명

일반 설명

Protein tyrosine phosphatase, catalytic domain, (amino acids 1-321), (Gene bank accession no. M31724) with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

Protein tyrosine phosphatase, non-receptor type 1 (PTPN1), which is also known as protein-tyrosine phosphatase 1B (PTP1B), is a tyrosine phosphatase. It is localized at the cytoplasmic side of the endoplasmic reticulum. The gene encoding PTPN1 is localized on human chromosome 20q13.13.

애플리케이션

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

생화학적/생리학적 작용

Protein tyrosine phosphatase, non-receptor type 1 (PTPN1) or protein-tyrosine phosphatase 1B (PTP1B) dephosphorylates the epidermal growth factor receptor. It acts as a modulator of insulin-like growth factor receptor (IGFR) and MET-mediated signaling responses. It may be useful as a drug target in type 2 diabetes and obesity. The protein has been shown to enhance tumor progression in various cancers such as breast, colon and prostate. It acts a tumor suppressor in other cancers (lymphomas and esophageal cancers).

단위 정의

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

물리적 형태

Formulated in 25 Mm Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable