SEQXE
SeqPlex DNA Amplification Kit
For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.
동의어(들):
SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit
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모든 사진(5)
About This Item
UNSPSC 코드:
41121800
NACRES:
NA.55
추천 제품
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일반 설명
SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.
애플리케이션
SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.
특징 및 장점
- Random priming technology amplifies fragmented DNA such as ChIP or FFPE
- Facilitates sequencing from as little as 100 pg of ChIP DNA
- Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
- Compatible with Illumina®, SOLiD™, or 454 library prep for next generation sequencing
기타 정보
SEQXE-500RXN is manufactured on-demand. Contact technical services at techserv@sial.com for more information.
법적 정보
Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
키트 구성품 역시 별도로 이용 가능함
제품 번호
설명
SDS
- W4502Water, Nuclease-Free Water, for Molecular BiologySDS
- Library Preparation Buffer
필요하나 제공되지 않음
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Joshua A Udall et al.
Frontiers in plant science, 10, 1541-1541 (2019-12-13)
One of the extraordinary aspects of plant genome evolution is variation in chromosome number, particularly that among closely related species. This is exemplified by the cotton genus (Gossypium) and its relatives, where most species and genera have a base chromosome
Takuya Hayakawa et al.
Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
E/L Repli-seq is a powerful tool for detecting cell type-specific replication landscapes in mammalian cells, but its potential to monitor DNA replication under replication stress awaits better understanding. Here, we used E/L Repli-seq to examine the temporal order of DNA
Optimizing sparse sequencing of single cells for highly multiplex copy number profiling.
Baslan T, et al.
Genome Research, 25(5), 714-724 (2015)
SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.
Mian S A, et al.
Nature Communications, 6, 10004-10004 (2015)
프로토콜
The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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