SAE0049

L-Lactate Dehydrogenase (LDHA)

from human, recombinant, expressed in E. coli, aqueous solution

• 동의어(들): Lactic Dehydrogenase, recombinant from E. coli, α-HBDH, α-hydroxy butyratede hydrogenase, anaerobic lactate dehydrogenase, (S)-Lactate: NAD⁺ oxidoreductase, L-Lactate Dehydrogenase, Lactate
• CAS Number: 9001-60-9
• 효소 위원회 번호: 1.1.1.27 (BRENDA, IUBMB)
• MDL number: MFCD00131466
• UNSPSC 코드: 12352200
• NACRES: NA.54

속성

• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli
• 양식: aqueous solution
• 저장 조건: (Keep container tightly closed in a dry and well-ventilated place)
• 색상: colorless
• UniProt 수납 번호: K1T0A2
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... LDHA(3939)

설명

일반 설명

Research area: Cell Signaling
The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15. It is a subunit of lactate dehydrogenase.In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.

애플리케이션

L-Lactate Dehydrogenase (LDHA) has been used in in vitro phosphoglycerate mutase 1 (PGAM1) inhibitors screening assay. It has also been used in a colorimetric assay for determining lactate concentration in conditioned media.

생화학적/생리학적 작용

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.  L-lactate dehydrogenase A chain (LDHA), an enzyme involved in pyruvate metabolism, LDH is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. LDHA regulates the microenvironment of developing tumors by the hypoxia-inducible factor (HIF)-signaling pathway. LDHA aids in the NAD⁺ regeneration during the β-oxidation of fatty acid. LDHA (L-lactate dehydrogenase A chain) is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. In cancer cells, HIF-1a (hypoxia-inducible factor) induces the expression of LDHA, which helps in maintaining glycolysis in cells.

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.

단위 정의

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

물리적 형태

Buffered aqueous solution with Hepes (pH 7.5), NaCl and glycerol.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable