SAB4200384

Anti-SMAD6 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 동의어(들): Anti-MADH6, Anti-MADH7, Anti-SMAD family member 6
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~60 kDa
• 종 반응성: human, mouse
• 농도: ~1.0 mg/mL
• 기술: immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over-expressing human SMAD6; indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells; western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD6
• UniProt 수납 번호: O43541
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SMAD6(4091) ; rat ... Smad6(367100)

설명

일반 설명

SMAD6 is a member of inhibitory Smads (I-Smad) family. It consists of two conserved domains, the N-terminal MH1 and C-terminal MH2, that are separated by a proline-rich linker region of varying length.

면역원

peptide corresponding to the N-terminal region of human SMAD6, conjugated to KLH. The corresponding sequence is identical in rat and differs by a single amino acid in mouse.

애플리케이션

Anti-SMAD6 (N-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

생화학적/생리학적 작용

SMAD6 plays an important role in transforming growth factor-β (TGF-β) pathway. It regulates cell proliferation, apoptosis and differentiation. SMAD6 is a key negative regulator of bone morphogenetic protein (BMP) signaling in the cytoplasm and in the nucleus. SMAD6 inhibits SMAD signaling in the nucleus by interacting with transcriptional repressors such as HOXC-8, class I histone deacetylases (HDACs) and transcriptional corepressor C-terminal binding protein (CtBp).

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable