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Merck
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Key Documents

RAB0500

Sigma-Aldrich

Human p53 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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About This Item

UNSPSC 코드:
41116158
NACRES:
NA.32

종 반응성

human

포장

kit of 96 wells (12 strips x 8 wells)

기술

ELISA: suitable
capture ELISA: suitable

입력

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.3 ng/mL
standard curve range: 0.328-80 ng/mL

검출 방법

colorimetric

배송 상태

wet ice

저장 온도

−20°C

유전자 정보

human ... TP53(7157)

일반 설명

Cellular tumor antigen p53 is a tumor-suppressor protein that comprises transactivation at N-terminus, central sequence-specific DNA-binding domain, and regulatory domain at C-terminus. It plays a key role in autophagy regulation. It inhibits tumors and acts as a cellular stress sensor especially during nutrient deprivation, hypoxia, and ribosome dysfunction. The Human p-53 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human p-53 in serum, plasma, cell culture supernatants and urine.

면역원

Recombinant Human p53

애플리케이션

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human p53 ELISA Kit has been used for quantifying p53 using enzyme-linked immunosorbent assay (ELISA) in human myeloma RPMI-8226 cells, human colorectal cancer HCT-116 cells, human breast cancer MCF-7 cells and human leukemiaHL-60 cells.

기타 정보

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABELADDELISA 5X Assay/Sample Diluent Buffer D (Item K)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

픽토그램

Corrosion

신호어

Warning

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Walaa Hamada Abd-Allah et al.
Drug development research, 80(7), 933-947 (2019-07-26)
Novel 1,1-disubstituted cyclohexane-1-carboxamides 6a-h, 7a-e, and 8a-b were designed and synthesized as apoptotic inducers. Cytotoxicity test revealed that some compounds have strong to moderate effect, while others displayed weak action against different cancer cell lines including, MCF-7, HepG2, A549, and
Evaluation of roscovitine anticancer agent as a reference compound for cancer and apoptosis studies
Taha H, et al.
JIMD Reports null
Mohammed K Abd Elhameid et al.
Chemical & pharmaceutical bulletin, 66(10), 939-952 (2018-08-17)
A new series of pyridine and pyrimidine derivatives is designed and synthesized as potential antitumor molecules. The tested compounds show promising in vitro cytotoxic activity against HL-60 cell line as eight compounds: 4, 6, 11, 13, 14, 15, 18 and
p53 at a glance.
Colleen A Brady et al.
Journal of cell science, 123(Pt 15), 2527-2532 (2010-10-14)
Mohammed K Abd El Hameid et al.
Bioorganic chemistry, 86, 609-623 (2019-02-27)
The work representing the design and the cytotoxic screening of synthetic small molecules (SSMs) such as carbonitriles 3a-c, carboximidamides 4a-c, and oxadiazoles 5-19 as antitumor molecules. Molecules 4c, 9, 12, and 14 show promising cytotoxicity profiles against two cell lines

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