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Merck
모든 사진(1)

주요 문서

R0883

Sigma-Aldrich

RPMI-1640 Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

동의어(들):

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
UNSPSC 코드:
12352207
NACRES:
NA.75

제품명

RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

무균

sterile-filtered

양식

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

애플리케이션

RPMI-1640 Medium has been used:
  • to culture PC-3 prostate cell line and Jurkat cells
  • to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line
  • to cultivate circulating tumor cells (CTCs)

재구성

Supplement with 0.3 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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