D5671

DMEM - high glucose

With sodium Bicarbonate, without ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

• 동의어(들): DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
• UNSPSC 코드: 12352207
• NACRES: NA.75

속성

• 제품명: Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma
• Quality Level: 400
• 무균: sterile-filtered
• 양식: liquid
• 기술: cell culture | hybridoma: suitable; cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• 성분: L-glutamine: no; glucose: high; NaHCO₃: yes; sodium pyruvate: no; phenol red: yes; HEPES: no
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

애플리케이션

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

재구성

Supplement with 0.584 g/L L-glutamine.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable