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Merck
모든 사진(1)

주요 문서

PP0540

Sigma-Aldrich

GlycoProfile β-Elimination Kit

Sufficient for 24 samples

동의어(들):

β-Elimination Glycan Kit, Glycan Removal Kit

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.28

결합

(O-linked)

저장 온도

room temp

애플리케이션

The GlycoProfile β-Elimination kit contains a novel non-reducing reagent to efficiently and specifically remove O-linked carbohydrates from glycoproteins with minimal protein or glycan destruction. Consequently, additional downstream proteomics and glycomic analyses may be employed.

수량

The kit includes the reagents and techware for O-deglycosylation of up to 24 samples (200 uL at 1-10 ug/uL of glycoprotein per sample).

법적 정보

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

키트 구성품 전용

제품 번호
설명

  • Sodium hydroxide solution, 5.0 M 60 μL

  • Microcon centrifugal filter unit, YM-10 membrane, NMWCO 10 kDa 24 ea

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS

  • Centrifugal Filter Unit

픽토그램

FlameCorrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

3 - Flammable liquids

Flash Point (°F)

82.0 °F - closed cup

Flash Point (°C)

27.79 °C - closed cup


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Prediction of glycoprotein secondary structure using ATR-FTIR
Lewis, S.P., et al.
Vibrational Spectroscopy, 69, 21-29 (2013)

문서

Our's GlycoProfile Beta-Elimination Kit allows researchers to perform complete glycoproteomics research by preserving both the O-glycans and protein, specifically remove o-glycans, label o-glycans prior to analysis, have confidence in uniformity of procedure. While it is known that O-glycosylation plays a major role in regulatory biology (as evident with studies around O-GlcNac and RNAi knockdowns of glycosyltransferases) the development of methods for the study of O-glycosylation is under represented compared to N-glycosylation. The under representation is primarily due to the difficulty in removing O-glycans while keeping both the protein and glycans intact. In contrast to N-glycosylation, there is no single enzyme capable of complete O-deglycosylation, so chemical methods must be employed.

Our's GlycoProfile Beta-Elimination Kit allows researchers to perform complete glycoproteomics research by preserving both the O-glycans and protein, specifically remove o-glycans, label o-glycans prior to analysis, have confidence in uniformity of procedure. While it is known that O-glycosylation plays a major role in regulatory biology (as evident with studies around O-GlcNac and RNAi knockdowns of glycosyltransferases) the development of methods for the study of O-glycosylation is under represented compared to N-glycosylation. The under representation is primarily due to the difficulty in removing O-glycans while keeping both the protein and glycans intact. In contrast to N-glycosylation, there is no single enzyme capable of complete O-deglycosylation, so chemical methods must be employed.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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