콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

P9041

Sigma-Aldrich

Phosphodiesterase II from bovine spleen

lyophilized powder, ≥5.0 units/mg protein

동의어(들):

3′-Exonuclease, Spleen exonuclease, Spleen phosphodiesterase

로그인조직 및 계약 가격 보기


About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

가격 및 재고 정보를 현재 이용할 수 없음

형태

lyophilized powder

Quality Level

특이 활성도

≥5.0 units/mg protein

분자량

65 kDa

UniProt 수납 번호

외래 활성

5′-Nucleotidase ≤1% of base activity

저장 온도

−20°C

유전자 정보

유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목
T2269137254T8566
L-Tyrosine from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 99.0-101.0%

T8566

L-Tyrosine

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

-

assay

99.0-101.0%

product line

BioReagent

product line

-

product line

EMPROVE® EVOLVE

product line

BioXtra

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-30°C

storage temp.

room temp

color

white to beige

color

white to beige

color

-

color

white to off-white

일반 설명

Research area: Cell signaling. Phosphodiesterase (PDE) II belongs to the PDE superfamily and includes subtypes from one to 11.[1] They exist as a dimer and comprise of N-terminal regulatory domain, C-terminal prenylated domain, and a Zn2+containing catalytic domain.[1] The bovine spleen PDE2 corresponds to a molecular weight of 65 kDa. It requires divalent cations for its enzymatic activity and has an optimum pH of 7.5.[2]

애플리케이션

Phosphodiesterase (PDE) is any enzyme that is used to break phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase II has been used in the enzymatic digestions of purified proteins such as the P8-dGMP complex[3]. Bovine spleen phosphodiesterase has been used to digest N-cadherin[4]. Phosphodiesterase II from bovine is suitable for sequential and end-group analysis of poly and oligonucleotides. It may be used in kinetic studieson substrate degradation of macro-molecules.
Phosphodiesterase II from bovine spleen has been used in the:
  • excision of pyridyloxobutyl (POB) base adducts from DNA[5]
  • digestion of DNA prior to cycloadenosine enrichment[6]
  • hydrolysis of DNA from blue mussels gill tissue into deoxyribonucleoside 3′-monophosphates[7]

The product has been used in the characterization of polynucleotide chain length, base composition, and identity of terminal nucleotide. The enzyme has also been used in excision of pyridyloxobutyl (POB) base adducts from DNA.[5] Furthermore, it has been used along with micrococcal endonuclease to hydrolyze purified DNA to 3 -nucleoside monophosphates.[8]

생화학적/생리학적 작용

Phosphodiesterase (PDE) breaks phosphodiester bonds.[9] 3-Isobutyl-methylxanthine (IBMX) is a potential PDE2 inhibitor.[10]
The enzyme acts on poly(A), poly(U), and poly(I). Native DNA and poly(C) are quite resistant to the action of this enzyme.
Hydrolyzes RNA, RNA-Core, 3′-alkyl- and 3′-aryl-nucleoside phosphates, and polydeoxyribonucleotides with 3′-phosphate end groups to 3′-mononucleotides.
Polynucleotides having 5′-phosphomonoester end groups are not attacked.

단위 정의

One unit will produce acid soluble nucleotides equivalent to a ΔA260 of 16 in 30 min at pH 6.5 at 37 °C, in a 2.0 mL reaction mixture. Substrate: RNA-Core. Actual A260 is measured on the supernatant after precipitation of the unhydrolyzed RNA with uranyl acetate-perchloric acid reagent.

분석 메모

Protein determined by biuret

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

샘플 COA가 필요하신가요?

이는 샘플 분석 증명서(COA)이며 이 특정 제품의 최근 제조된 로트를 대표하지 않습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

이미 열람한 고객

Slide 1 of 1

1 of 1

Phosphodiesterase inhibitors, congestive heart failure, and sudden death: Time for re-evaluation
Varma A, et al.
Congestive Heart Failure (Greenwich, Conn.), 18(4), 229-233 (2012)
Rajesh Gupta et al.
Journal of inorganic biochemistry, 99(2), 552-559 (2004-12-29)
A combination of enzymatic digestion and electrospray ionisation mass spectrometry (ESI-MS) was used to characterise bifunctional adducts in which cisplatin is bound to GA base sequences in 8mer and 16mer oligonucleotides that do not contain other, higher affinity binding sites.
María Jesús Juan-Fita et al.
European journal of pharmacology, 512(2-3), 207-213 (2005-04-21)
The effects of phosphodiesterase (PDE) inhibitors (1-3) on tissue cAMP concentrations and the inotropic responses to dobutamine and glucagon were investigated in electrically driven right ventricular strips of the rat heart. Dobutamine (0.3-100 microM) produced a concentration-dependent positive inotropic effect
Hisao Saneyoshi et al.
The Journal of organic chemistry, 70(25), 10453-10460 (2005-12-06)
[reaction: see text] An effective method for the synthesis of 2'-O-cyanoethylated oligoribonucleotides as a new class of 2'-O-modified RNAs was developed. The reaction of appropriately protected ribonucleoside derivatives with acrylonitrile in t-BuOH in the presence of Cs2CO3 gave 2'-O-cyanoethylated ribonucleoside
J Balsamo et al.
The Journal of biological chemistry, 265(5), 2923-2928 (1990-02-15)
Calcium-dependent cell-cell adhesion among embryonic chick neural retina cells is mediated by N-cadherin, a 130,000-Da integral membrane protein. We have reported that the ability of chick neural retina cells to form calcium-dependent cell-cell adhesion is also correlated with the presence

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.