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Merck
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Key Documents

P7998

Sigma-Aldrich

Alkaline Phosphatase Yellow

alkaline phosphatase substrate, chromogenic liquid

동의어(들):

Alkaline phosphatase substrate solution (pNPP)

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About This Item

UNSPSC 코드:
12352204
eCl@ss:
42010105
NACRES:
NA.83

product name

Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA, ready to use solution

Quality Level

형태

liquid

저장 온도

2-8°C

일반 설명

The product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications.

애플리케이션

Develops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications.

주의사항

 Stable at least one year at 2-8 °C.

물리적 형태

Ready to use

제조 메모

Contains p-nitrophenyl phosphate, a substrate for alkaline phosphatase.

픽토그램

Health hazardCorrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Cristina A Teixeira et al.
Clinical science (London, England : 1979), 130(18), 1665-1675 (2016-07-08)
Transthyretin (TTR)-related amyloidoses are diseases characterized by extracellular deposition of amyloid fibrils and aggregates in tissues composed of insoluble misfolded TTR that becomes toxic. Previous studies have demonstrated the ability of small compounds in preventing and reversing TTR V30M deposition
Laura C Jacques et al.
Nature communications, 11(1), 1892-1892 (2020-04-22)
Streptococcus pneumoniae serotype 1 is the predominant cause of invasive pneumococcal disease in sub-Saharan Africa, but the mechanism behind its increased invasiveness is not well understood. Here, we use mouse models of lung infection to identify virulence factors associated with
Hannah M Rowe et al.
Infection and immunity, 87(10) (2019-07-17)
Acute otitis media is one of the most common childhood infections worldwide. Currently licensed vaccines against the common otopathogen Streptococcus pneumoniae target the bacterial capsular polysaccharide and confer no protection against nonencapsulated strains or capsular types outside vaccine coverage. Mucosal
Molhm Nassir et al.
Organic & biomolecular chemistry, 17(46), 9913-9923 (2019-11-14)
Nucleotide pyrophosphatase/phosphodiesterase-1 (NPP1) inhibitors have been suggested as a potential treatment for calcium pyrophosphate dihydrate (CPPD) deposition disease. Here, we targeted the development of improved NPP1 inhibitors based on acyclic mimics of Pα,α-phosphorodithioate-substituted adenine nucleotides, 7-10. The latter were obtained
Ortal Danino et al.
Rheumatology (Oxford, England), 57(8), 1472-1480 (2018-04-25)
Calcium pyrophosphate deposition (CPPD) is associated with osteoarthritis and is the cause of a common inflammatory articular disease. Ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (eNPP1) is the major ecto-pyrophosphatase in chondrocytes and cartilage-derived matrix vesicles (MVs). Thus, eNPP1 is a principle contributor to

문서

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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