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Merck
모든 사진(1)

주요 문서

N1891

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST, chromogenic, tablet

동의어(들):

pNPP tablets

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About This Item

MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.83

제품명

SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL

Quality Level

양식

tablet

solubility

deionized water: soluble 5 mL

저장 온도

−20°C

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애플리케이션

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
SIGMA FAST p-Nitrophenyl phosphate Tablets has been used:
  • to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2)
  • to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay
  • as a substrate for ExtrAvidin® alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA)
  • as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin

재구성

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.

법적 정보

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서

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