P4334

Anti-Protein Kinase Cα antibody produced in rabbit

whole antiserum

• 동의어(들): Anti-PKCα
• MDL number: MFCD00162769
• UNSPSC 코드: 12352203
• NACRES: NA.44

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: whole antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 분자량: antigen 80 kDa
• 포함: 15 mM sodium azide
• 종 반응성: mouse, rat
• 기술: microarray: suitable; western blot: 1:20,000 using mouse NIH 3T3 fibroblast lysate; western blot: 1:50,000 using rat brain extract
• UniProt 수납 번호: P05696
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Prkca(18750) ; rat ... Prkca(24680)

설명

일반 설명

Anti-Protein Kinase Cα is produced in rabbit using a synthetic peptide conjugated to keyhole limpet hemocyanin (KLH) as the immunogen. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors, and neurotransmitters.

PKC α is a conventional isoform of Protein Kinase C (76-93 kD) and a member of serine/threonine (Ser/Thr) protein kinases. It is expressed ubiquitously in tissues and is the major isoform of PKC in fibroblasts. Stimulation and overexpression of PKC α increases the growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues. It is also useful for detecting PKC α isoenzyme in brain tissue and cell extracts. Furthermore, it can be used in chemiluminescence detection systems to detect PKC α. Rabbit anti-protein kinase c α antibody reacts specifically with PKC α (80 kD) of rat brain extract or NIH3T3 mouse fibroblast lysate.

특이성

The antibody reacts in immunoblotting with PKCα in rat brain extract. Staining is specifically inhibited with PKCα immunizing peptide (659-672).

면역원

Synthetic peptide corresponding to amino acids from the C-terminal variable (V5) region of rat PKC α conjugated to KLH.

애플리케이션

Anti-Protein Kinase C α has been used in immunoblotting and immunohistochemistry.

Anti-Protein Kinase Cα antibody produced in rabbit has been used in light microscopic—immunocytochemical analysis and immunofluorescent labelling.

생화학적/생리학적 작용

Protein Kinase C (PKC) plays a crucial role in signal transduction leading to cellular regulation, cell growth and differentiation, oncogenesis and modulation of neurotransmission. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced as a result from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors and neurotransmitters. PKC is also the major cellular receptor for the tumor promoting phorbol esters. PKC action is mediated by binding to specific receptors for activated C-kinase (RACKs) and through the phosphorylation of several cellular substrates. Overexpression and stimulation of PKC α leads to enhanced growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues.

물리적 형태

Supplied as a liquid containing 15 mM sodium azide as preservative

저장 및 안정성

For continuous use, store at 2-8 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable