추천 제품
애플리케이션
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.
Phosphatase, Alkaline from Escherichia coli has been used:
- in fluorometric assay to determine its enzyme activity
- in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
- in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity
생화학적/생리학적 작용
Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
주의사항
Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.
단위 정의
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.
물리적 형태
Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl2, 0.5 mM ZnCl2, 50% (w/v) glycerol
분석 메모
Protein determined by biuret.
기질
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
Phospho-iTRAQ data article: Assessing isobaric labels for the large-scale study of phosphopeptide stoichiometry
Glibert P, et al.
Data in Brief, 4(2), 60-65 (2015)
Sensitive fluorogenic substrate for alkaline phosphatase
Levine MN and Raines RT
Analytical Biochemistry, 418(2), 247-252 (2011)
The prion protein and cellular cholesterol homeostasis
Diomede L, et al.
Neurobiology of Lipids, 1(2), 8-14 (2002)
Lionel M Igaz et al.
The American journal of pathology, 173(1), 182-194 (2008-06-07)
TAR DNA-binding protein (TDP-43) has been recently described as a major pathological protein in both frontotemporal dementia with ubiquitin-positive inclusions (FTLD-U) and amyotrophic lateral sclerosis. However, little is known about the relative abundance and distribution of different pathological TDP-43 species
Jong Kil Lee et al.
The Journal of experimental medicine, 211(8), 1551-1570 (2014-07-23)
In Alzheimer's disease (AD), abnormal sphingolipid metabolism has been reported, although the pathogenic consequences of these changes have not been fully characterized. We show that acid sphingomyelinase (ASM) is increased in fibroblasts, brain, and/or plasma from patients with AD and
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