N3393

Naphthol Blue Black

BioReagent, suitable for electrophoresis

• 동의어(들): Acid Black 1, Amido Black 10B, Buffalo Black NBR
• 실험식(Hill 표기법): C₂₂H₁₄N₆Na₂O₉S₂
• CAS Number: 1064-48-8
• Molecular Weight: 616.49
• 색상 지수 번호: 20470
• Beilstein: 5374263
• EC Number: 213-903-1
• MDL number: MFCD00004017
• UNSPSC 코드: 41105322
• PubChem Substance ID: 24897642
• NACRES: NA.32

속성

• 제품 라인: BioReagent
• 형태: powder
• 구성: Dye content, ~85%
• 기술: protein staining: suitable
• 불순물: ≤10% water
• 적합성: suitable for electrophoresis
• SMILES string: [Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
• InChI key: AOMZHDJXSYHPKS-DROYEMJCSA-L

설명

애플리케이션

Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.

생화학적/생리학적 작용

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1B
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves