추천 제품
사용
sufficient for 100 colorimetric tests
Quality Level
검출 방법
colorimetric
관련 질환(들)
gastrointestinal diseases
저장 온도
−20°C
일반 설명
Trypsin is a pancreatic serine protease, which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. Tryptic digestion of the protein of interest results in a highly specific cleavage and a limited number of peptide fragments. In this assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at λ= 405 nm. Trypsin activity can be accurately measured by the color intensity which is proportional to p-NA content. The kit detects 10–100 mU (p-NA unit) trypsin in various samples.
특징 및 장점
Highly sensitive, simple and high-throughput assay for measuring Trypsin concentration.
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
적합성
Suitable for the detection of Trypsin in various biological samples such as cell and tissue extracts, serum, and plasma.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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