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Merck
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Key Documents

MAK450

Sigma-Aldrich

Glucose Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

동의어(들):

GDH Activity Assay Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

Quality Level

저장 조건

(No metal containers. Keep container tightly closed in a dry and well -ventilated place. Keep away from heat and sources of ignition.)

입력

tissue
cell culture medium
tissue
plasma

검출 방법

colorimetric

관련 질환(들)

endocrinological disorders, diabetes; hematological disorder

저장 온도

−20°C

일반 설명

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

애플리케이션

The Glucose Dehydrogenase Assay Kit may be used for:
  • Metabolism Research
  • Drug Discovery

특징 및 장점

  • Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose dehydrogenase levels across a broad range from 0.5 to 200 U/L, providing both convenience and sensitivity.
  • Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

적합성

The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

원리

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

픽토그램

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신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

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