추천 제품
일반 설명
The new Fumarase Assay Kit, MAK521, is now available! Fumarase (Fumarate Hydratase; EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (Krebs) cycle. The activity of fumarase is important as it produces reducing equivalents that drive the synthesis of ATP and maintains energy balance in aerobic metabolism. A deficiency of fumarase activity results in progressive encephalopathy.
애플리케이션
Fumarase Activity Colorimetric Assay Kit has been used to determine fumarase (FUM) activity.
적합성
Suitable for the detection of Fumarase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
원리
The Fumarase Activity Colorimetric Assay Kit provides a simple and high-throughput ready procedure for measuring fumarase activity in a variety of tissues, cells, and isolated mitochondria. Fumarase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of fumarase is the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 9.5 at 37 °C.
키트 구성품 전용
제품 번호
설명
- Fumarase Assay Buffer
- Fumarase Substrate
- Fumarase Enzyme Mix
- Fumarase Developer
- NADH Standard
- Fumarase Positive Control
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
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시험 성적서(COA)
Lot/Batch Number
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Li L, et al.
The Plant Journal, 89(3), 458-471 (2017)
Lei Li et al.
The Plant journal : for cell and molecular biology, 89(3), 458-471 (2016-10-12)
Mitochondrial Lon1 loss impairs oxidative phosphorylation complexes and TCA enzymes and causes accumulation of specific mitochondrial proteins. Analysis of over 400 mitochondrial protein degradation rates using 15 N labelling showed that 205 were significantly different between wild type (WT) and
Helena Hernández-Cuervo et al.
Frontiers in physiology, 13, 814510-814510 (2022-04-19)
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Ping Li et al.
International journal of molecular sciences, 23(3) (2022-02-16)
The FH gene encodes the fumarate hydratase of the Krebs cycle and functions as a homotetramer to catalyze the hydration of fumarate to malate. Mutations in FH result in uterine leiomyomas, a rare autosomal dominant inherited metabolic disease. However, how
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