MAK189
A-KG Dehydrogenase Activity Assay Kit
Sufficient for 100 Colorimetric tests
동의어(들):
α-KG Dehydrogenase Activity Assay Kit, α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, AKGDH Assay Kit, Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, OGDC Activity Assay Kit, Oxoglutarate Dehydrogenase Assay Kit
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모든 사진(2)
About This Item
UNSPSC 코드:
12161503
NACRES:
NA.84
추천 제품
검출 방법
colorimetric
관련 질환(들)
endocrinological disorders, diabetes; orthopedic diseases; neurological disorders; rheumatological diseases; cancer; cardiovascular diseases
저장 온도
−20°C
유전자 정보
human ... OGDH(4967)
mouse ... OGDH(18293)
rat ... OGDH(360975)
일반 설명
α-Ketoglutarate Dehydrogenase (α-KGDH, EC 1.2.4.2) is a highly regulated Krebs cycle enzyme, forms the E1 subunit of the mitochondrial 2-oxoglutarate dehydrogenase complex. It catalyzes the conversion of α-ketoglutarate to succinyl-CoA and produces NADH directly providing electrons for the respiratory chain. Decreased α-KGDH complex activity has been associated with oxidative stress and neurodegenerative disorders such as Alzheimer′s and Parkinson′s diseases.
애플리케이션
α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of α-ketoglutarate dehydrogenase in samples.
적합성
Suitable for the detection of α-ketoglutarate dehydrogenase activity in samples such as tissue, cells, and purified mitochondria.
원리
The α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit provides a simple procedure for measuring α-ketoglutarate dehydrogenase activity in a variety of tissues, cells, and isolated mitochondria. α-KGDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of α-ketoglutarate dehydrogenase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.5 at 37 °C.
키트 구성품 전용
제품 번호
설명
- KGDH Assay Buffer
- KGDH Substrate
- KGDH Developer
- NADH Standard
- KGDH Positive Control
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer.
Paredes F, et al.
Proceedings of the National Academy of Sciences of the USA, 115(38), 201720693-201720693 (2018)
Sirtuin lipoamidase activity is conserved in bacteria as a regulator of metabolic enzyme complexes.
Rowland E A, et al.
mBio, 8(5), e01096-e01017 (2017)
Alpha-ketoglutarate dehydrogenase: a target and generator of oxidative stress.
Tretter L and Vera A
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 360(1464), 2335-2345 (2005)
Alexandre Buffet et al.
The Journal of clinical endocrinology and metabolism, 106(2), 459-471 (2020-11-13)
Pheochromocytomas and paragangliomas (PPGLs) are neuroendocrine tumors in which altered central metabolism appears to be a major driver of tumorigenesis, and many PPGL genes encode proteins involved in the tricarboxylic acid (TCA) cycle. While about 40% of PPGL cases carry
Elizabeth A Rowland et al.
mBio, 8(5) (2017-09-14)
Lipoic acid is an essential metabolic cofactor added as a posttranslational modification on several multimeric enzyme complexes. These protein complexes, evolutionarily conserved from bacteria to humans, are core regulators of cellular metabolism. While the multistep enzymatic process of adding lipoyl
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