추천 제품
사용
sufficient for 100 colorimetric tests
응용 분야
cosmetics
environmental
food and beverages
검출 방법
colorimetric
관련 질환(들)
endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases; rheumatological diseases
저장 온도
2-8°C
일반 설명
Oxidants, such as reactive oxygen species (ROS) and reactive nitrogen species (RNS), can generate free radicals that can cause severe oxidative damage to cellular lipids, membranes, proteins, and DNA. Antioxidants can scavenge these free radicals and prevent cellular oxidative stress by enzymatic andnon-enzymatic mechanisms. Enzyme systems that function as antioxidants include catalase and peroxidase. Tocopherols, carotenes, vitamin A, and ubiquinols function as lipid-soluble antioxidants; whereas, glutathione and ascorbate are some of the water-soluble antioxidants. Measurement of the total non-enzymatic antioxidant capacity (TAC) of biological samples is indicative of their ability to counteract oxidative stress-induced damage in cells. TAC is used to provide insights into the development and treatment of oxidative-stress related disorders.
In the Total Antioxidant Capacity Assay Kit, either the concentration of the combination of both small molecule and protein antioxidants, or the concentration of only small molecule antioxidants can be determined. Cu2+ ion is converted to Cu+ by both small molecules and proteins. However, the use of the Protein Mask prevents Cu2+ reduction by protein, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion chelates with a colorimetric probe, giving a broad absorbance peak at ~570 nm, which is proportional to the total antioxidant capacity. The kit gives antioxidant capacity in Trolox equivalents (ranging from 4-20 nmole/well). Trolox, a water-soluble vitamin E analog, serves as an antioxidant standard.
In the Total Antioxidant Capacity Assay Kit, either the concentration of the combination of both small molecule and protein antioxidants, or the concentration of only small molecule antioxidants can be determined. Cu2+ ion is converted to Cu+ by both small molecules and proteins. However, the use of the Protein Mask prevents Cu2+ reduction by protein, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion chelates with a colorimetric probe, giving a broad absorbance peak at ~570 nm, which is proportional to the total antioxidant capacity. The kit gives antioxidant capacity in Trolox equivalents (ranging from 4-20 nmole/well). Trolox, a water-soluble vitamin E analog, serves as an antioxidant standard.
애플리케이션
Total antioxidant capacity assay kit has been used to determine total antioxidant capacity of leaf tissue samples.
적합성
This kit is suitable for the detection of small molecule and protein antioxidants in cell and tissue lysates, media, or in biological fluids such as urine, plasma, and serum.
원리
In the Total Antioxidant Capacity Assay Kit, either the concentration of the combination of both small molecule and protein antioxidants, or the concentration of only small molecule antioxidants can be determined. Cu2+ ion is converted to Cu+ by both small molecules and proteins. However, the use of the Protein Mask prevents Cu2+ reduction by protein, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion chelates with a colorimetric probe, giving a broad absorbance peak at ~570 nm, which is proportional to the total antioxidant capacity. The kit gives antioxidant capacity in Trolox equivalents (ranging from 4-20 nmole/well). Trolox, a water-soluble vitamin E analog, serves as an antioxidant standard.
키트 구성품 전용
제품 번호
설명
- Cu2+ Reagent
- Assay Diluent
- Protein Mask
- Trolox Standard, 1 μmole
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1
Storage Class Code
10 - Combustible liquids
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Free radicals: properties, sources, targets, and their implication in various diseases.
Phaniendra A, et al.
Indian Journal of Clinical Biochemistry : IJCB, 30(1), 11-26 (2015)
UV-B antagonises shade avoidance and increases levels of the flavonoid quercetin in coriander (Coriandrum sativum).
Fraser D P, et al.
Scientific Reports, 7(1), 17758-17758 (2017)
Oxidative Stress and Antioxidant Defense.
Esra B, et al.
The World Allergy Organization journal, 5(1), 9-19 (2012)
Toni Tamminen et al.
Oxidative medicine and cellular longevity, 2021, 8028427-8028427 (2021-12-18)
Chronic oxidative stress eventually leads to protein aggregation in combination with impaired autophagy, which has been observed in age-related macular degeneration. We have previously shown an effective age-related macular degeneration disease model in mice with nuclear factor-erythroid 2-related factor-2 (NFE2L2)
Niclas U Lundsgaard et al.
Conservation physiology, 8(1), coaa002-coaa002 (2020-05-30)
Multiple environmental changes are thought to be contributing to the widespread decline of amphibians in montane regions, but interactions between drivers of decline are not well understood. It has been proposed previously that elevated ultraviolet-B radiation (UBVR) and low temperatures
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