추천 제품
검출 방법
colorimetric
fluorometric
관련 질환(들)
immunological diseases; cardiovascular diseases
저장 온도
−20°C
유전자 정보
human ... EBI4(387712) , ENO1(2023) , ENO2(2026)
mouse ... EBI4(226265) , ENO1(13806) , ENO2(13807)
rat ... EBI4(292138) , ENO1(24333) , ENO2(24334)
일반 설명
Enolase is a multifunctional glycolytic enzyme that catalyzes the conversion of D-2-phosphoglycerate to phospho(enol)pyruvate (PEP) and water. It can function as a plasminogen receptor in endothelial, epithelial, and hematopoietic cells and hence, may be involved in fibrinolytic and intravascular systems. It is also known to act as a heat shock protein, which may have implications in transcriptional and pathological functions. Enolase has been implicated in autoimmune and systemic diseases. Furthermore; serum neuron-specific enolase is known to function as a predictor of patient outcome post cardiac arrest. Thus enolase assays can be used for studying cellular functions like carbohydrate metabolism, transcription and other pathophysiological processes.
애플리케이션
Enolase Activity Assay Kit has been used to measure enolase activity.
적합성
Suitable for the detection of enolase activity in biological samples.
원리
The Enolase activity is determined by a coupled enzyme assay in which D-2-phosphoglycerate is converted to PEP, resulting in the formation of an intermediate that reacts with a peroxidase substrate, generating a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product proportional to the enolase activity present. One milliunit of enolase is the amount of enzyme that will generate 1.0 nmole of H2O2 per minute at pH 7.2 at 25 °C.
키트 구성품 전용
제품 번호
설명
- Enolase Assay Buffer
- Peroxidase Substrate, in DMSO
- Enolase Substrate Mix
- Enolase Converter
- Enolase Developer
- Enolase Positive Control
- Hydrogen Peroxide Standard, 0.88 M
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Global Profiling of Lysine Acetylation in Borrelia burgdorferi B31 Reveals Its Role in Central Metabolism.
Bontemps-Gallo S, et al.
Frontiers in Microbiology, 9, 2036-2036 (2018)
WW domain-binding protein 2 acts as an oncogene by modulating the activity of the glycolytic enzyme ENO1 in glioma.
Chen S, et al.
Cell Death & Disease, 9(3), 347-347 (2018)
Jiayu Zhou et al.
Cell death & disease, 10(12), 885-885 (2019-11-27)
Lung adenocarcinoma (LUAD) has long been one of the predominant reasons for the global cancer-linked mortality. The tumor progression is shown by several studies to be promoted by increased glycolysis. Enolase 1 (ENO1), as a glycolysis enzyme, performs pivotal role
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Frontiers in microbiology, 9, 2036-2036 (2018-09-21)
The post-translational modification of proteins has been shown to be extremely important in prokaryotes. Using a highly sensitive mass spectrometry-based proteomics approach, we have characterized the acetylome of B. burgdorferi. As previously reported for other bacteria, a relatively low number
Xing Huang et al.
Autophagy, 15(7), 1258-1279 (2019-02-23)
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