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Merck
모든 사진(1)

Key Documents

MAK118

Sigma-Aldrich

Invertase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

동의어(들):

β-fructofuranosidase Assay Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric or fluorometric tests

응용 분야

cosmetics
environmental
food and beverages

검출 방법

colorimetric
fluorometric

저장 온도

−20°C

일반 설명

Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.

애플리케이션

Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.

특징 및 장점

Compatible with high-throughput handling systems.

적합성

Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.

원리

In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.

Storage Class Code

10 - Combustible liquids


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문서 라이브러리 방문

이미 열람한 고객

Identification and Functional Characterization of Sugarcane Invertase Inhibitor (ShINH1): A Potential Candidate for Reducing Pre-and Post-harvest Loss of Sucrose in Sugarcane.
Shivalingamurthy S G, et al.
Frontiers in Plant Science, 9 (2018)
Roua Gabriela Popescu et al.
Animals : an open access journal from MDPI, 10(5) (2020-05-14)
The purpose of this study was to examine the effects of dietary inclusion of two additives at the final concentration of 0.5% bilberry (E1) and 1% walnut (E2) leaves powder in the basal diet on digestive health of hens. A
Domenica Farci et al.
Journal of experimental botany, 67(11), 3303-3312 (2016-04-17)
Invertases are a widespread group of enzymes that catalyse the conversion of sucrose into fructose and glucose. Plants invertases and their substrates are essential factors that play an active role in primary metabolism and in cellular differentiation and by these
Khaled M Hazzouri et al.
Nature communications, 10(1), 4680-4680 (2019-10-17)
Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa. Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species. Here we report
Suresha G Shivalingamurthy et al.
Frontiers in plant science, 9, 598-598 (2018-05-19)
In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play

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