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Merck
모든 사진(3)

문서

MAK055

Sigma-Aldrich

AST Activity Assay Kit

sufficient for 100 colorimetric tests

동의어(들):

Aspartate Aminotransferase Activity Assay Kit, Glutamate-Oxaloacetate Transaminase Activity Assay Kit, SGOT Activity Assay Kit, Serum Glutamate-Oxaloacetate Transaminase Activity Assay Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric tests

검출 방법

colorimetric

관련 질환(들)

gastrointestinal diseases; cardiovascular diseases

저장 온도

−20°C

유전자 정보

human ... GOT1(2805)
mouse ... GOT1(14718)
rat ... GOT1(24401)

일반 설명

The new AST Assay Kit, MAK467, is now available! Aspartate Aminotransferase (AST), formerly called serum glutamate-oxaloacetate transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Similar to Alanine Aminotransferase (ALT), AST levels in blood are commonly used as a marker for liver function. However, AST has a broader tissue distribution than ALT and perturbations in AST levels can occur in response to diseases or injuries in multiple tissues including skeletal and heart.

애플리케이션

AST Activity Assay Kit has been used to determine the activity of aspartate aminotransferase in samples.

특징 및 장점

Compatible with high-throughput handling systems.

적합성

Suitable for the measurement of aspartate aminotransferase (AST) activity in biological samples including tissue, cells and serum

원리

The Aspartate Aminotransferase Activity Assay kit provides a simple and direct procedure for measuring AST activity in a variety of samples. In this kit, the transfer of an amino group from aspartate to α-ketoglutarate results in the generation of glutamate, resulting in the production of a colorimetric (450 nm) product proportional to the AST enzymatic activity present. One unit of AST is the amount of enzyme that will generate 1.0 μmole of glutamate per minute at pH 8.0 at 37 °C.

픽토그램

Health hazardCorrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials


시험 성적서(COA)

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문서 라이브러리 방문

Leola N Chow et al.
PloS one, 11(3), e0151765-e0151765 (2016-03-22)
Modulation of chemokine CXCL12 and its receptor CXCR4 has been implicated in attenuation of bleomycin (BLM)-induced pulmonary fibrosis and carbon tetrachloride (CCl4)-induced hepatic injury. In pulmonary fibrosis, published reports suggest that collagen production in the injured lung is derived from
Resolvin D1 and E1 alleviate the progress of hepatitis toward liver cancer in long-term concanavalin A-induced mice through inhibition of NF-KB activity.
Kuang H, et al.
Oncology Reports, 35, 307-317 (2016)
Xinyi Xu et al.
Microbiology (Reading, England), 161(6), 1219-1230 (2015-03-27)
Aspartate and glutamate are two key amino acids used in biosynthesis of many amino acids that play vital role in cellular metabolism. Aspartate aminotransferases (AspATs) are required for channelling nitrogen (N(2)) between Glu and Asp in all life forms. Biochemical
Zhiqiang Ye et al.
Journal of cellular biochemistry, 119(9), 7397-7405 (2018-05-19)
Liver ischemia/reperfusion (I/R) injury has high mortality due to the intense inflammatory process occurs in the liver. However, the pathological mechanism underlying I/R injury is still not clear. Recent works showed that circular RNAs play critical roles in many human
Yu Wu et al.
Molecular medicine reports, 14(6), 5281-5287 (2016-10-26)
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