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Key Documents

M5650

Sigma-Aldrich

Minimum Essential Medium Eagle

With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

동의어(들):

EMEM, MEM

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

product name

Minimum Essential Medium Eagle, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

무균

sterile-filtered

형태

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.

애플리케이션

Minimum Essential Medium Eagle has been used to culture:
  • HeLa cells
  • Madin-Darby canine kidney (MDCK) cells
  • chondrosarcoma cell line

This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

재구성

Supplement with 0.292 g/L L-glutamine.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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