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Merck
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Key Documents

M7145

Sigma-Aldrich

MEM Non-essential Amino Acid Solution (100×)

Without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

동의어(들):

MEM NEAA

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About This Item

UNSPSC 코드:
12352207
eCl@ss:
32160406
NACRES:
NA.75

product name

MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

Quality Level

무균

sterile-filtered

제품 라인

BioReagent

형태

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

저장 온도

2-8°C

일반 설명

Eagle′s minimum essential medium (MEM) is a synthetic culture medium, which is composed of balanced salt solutions, and amino acids and vitamins. These components are essential for the growth of cultured cells. MEM might be supplemented with non-essential amino acids, to make MEM non-essential amino acid solution. The non-essential amino acids include L-alanine, L-asparagine, L-aspartic acid, L-glycine, L-serine, L-proline and L-glutamic acid.

애플리케이션

Adding supplements of amino acids to media both stimulates growth and prolongs the viability of cells in culture. MEM Non-essential Amino Acid Solution supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
MEM Non-essential Amino Acid Solution has been used in Dulbecco′s minimum essential media for the maintenance of mammalian cells. It has been supplemented in medium prepared for oocyte maturation.

생화학적/생리학적 작용

Studies on hybridoma culture shows that addition of Eagle′s minimum essential medium (MEM) non-essential amino acid leads to enhanced membrane stability, during death phase of the culture. This also results in increased antibody secretion during death phase, and decreased lactate concentration.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: relevance to intracellular reactive oxygen species and glutathione contents.
Hashimoto S et al.
Molecular Reproduction and Development, 56, 520-520 (2000)
Ayako Suzuki et al.
Genome biology, 16, 66-66 (2015-04-19)
To understand the heterogeneous behaviors of individual cancer cells, it is essential to investigate gene expression levels as well as their divergence between different individual cells. Recent advances in next-generation sequencing-related technologies have enabled us to conduct a single-cell RNA-Seq
Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures
Fassnacht D
Biotechnology Letters, 19(1), 35-38 (1997)
Janis A Müller et al.
Medical microbiology and immunology, 206(2), 175-185 (2017-02-09)
Zika virus (ZIKV) is an emerging pathogen that causes congenital infections which may result in birth defects, such as microcephaly. Currently, no approved treatment or vaccination is available. ZIKV can be readily detected in cell culture where virally infected cells
Lei Xi Chen et al.
The Laryngoscope, 129(9), 2139-2146 (2019-03-19)
Surgical adhesives are increasingly used for vocal fold microsurgery to assist wound closure and reduce the risks of scar formation. Currently used vocal fold adhesives such as fibrin glue, however, have thus far not been found to promote wound closure

프로토콜

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

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