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Merck
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Key Documents

L9379

Sigma-Aldrich

Lectin from Phytolacca americana (pokeweed)

lyophilized powder

동의어(들):

PWM, Pokeweed mitogen

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About This Item

MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.32

무균

aseptically filled

Quality Level

형태

lyophilized powder

효능

≤0.3 μg/mL mitogenic activity

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.

애플리케이션

Lectin from Phytolacca americana (pokeweed) has been used:
  • as a medium supplement for mononuclear cell incubation, to induce lymphocyte proliferation
  • as a positive control in the study of the role of chemokine (C–C motif) ligand 2 (CCL2) in proliferation and apoptosis of decidual leukocytes
  • as a supplement in the RPMI (Roswell park memorial institute) 1640 medium for peripheral blood lymphocyte cultures

생화학적/생리학적 작용

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

물리적 형태

Partially purified TCA precipitate.
Essentially salt-free.

분석 메모

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


시험 성적서(COA)

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