추천 제품
Grade
for molecular biology
Quality Level
무균
non-sterile
형태
powder
구성
Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
기술
microbiological culture: suitable
응용 분야
agriculture
food and beverages
microbiology
저장 온도
room temp
적합성
nonselective for Escherichia coli
nonselective for coliforms
일반 설명
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
애플리케이션
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
특징 및 장점
Lennox EZMix powder with agar provides:
- A budget-friendly alternative to pre-poured plates
- Granulated, dust-free format for safer handling and faster mixing
- Convenient small package to eliminate weighing
- Easy scale-up using larger package sizes
- Standard formulation
제조 메모
1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.
재구성
Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.
법적 정보
Zeocin is a registered trademark of Cayla Sarl
관련 제품
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Sambrook, J., et al
Molecular Cloning: A Laboratory Manual, 2, 1-1 (1989)
Ausubel, F. M.
Current Protocols in Molecular Biology, 1, 3-3 (1994)
Alexis Kaushansky et al.
Nature protocols, 5(4), 773-790 (2010-04-03)
Protein microarrays provide an efficient way to identify and quantify protein-protein interactions in high throughput. One drawback of this technique is that proteins show a broad range of physicochemical properties and are often difficult to produce recombinantly. To circumvent these
Raymond Liang et al.
Cell stem cell, 26(3), 359-376 (2020-02-29)
Quiescence is a fundamental property that maintains hematopoietic stem cell (HSC) potency throughout life. Quiescent HSCs are thought to rely on glycolysis for their energy, but the overall metabolic properties of HSCs remain elusive. Using combined approaches, including single-cell RNA
문서
General protocols for growth of competent cells and their transformation (uptake of DNA).
프로토콜
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
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