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Key Documents

L7025

Sigma-Aldrich

LB Broth with agar (Lennox)

Tablet microbial growth medium

동의어(들):

LB Agar, LB agar

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.85

Grade

for molecular biology

Quality Level

무균

non-sterile

형태

tablet

기술

microbiological culture: suitable

응용 분야

food and beverages
microbiology

저장 온도

room temp

적합성

nonselective for Escherichia coli
nonselective for coliforms

일반 설명

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

애플리케이션

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

특징 및 장점

Lennox LB tablet with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Convenient small package to eliminate weighing
  • Fast preparation – no stirring required

성분

13.72g/L Agar
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents

재구성

Add each 1.68 g tablet to 48.3 mL water (no stirring required). Autoclave for 20 minutes at 121 °C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

법적 정보

Zeocin is a registered trademark of Cayla Sarl

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)
Shun Gen Huang et al.
BMC immunology, 14, 27-27 (2013-06-22)
Surgical intervention-related trauma contributes largely to the development of postoperative immunosuppression, with reduced resistance to secondary bacterial infection. This study compared the impact of laparotomy versus laparoscopy on macrophage-associated bactericidal ability and examined whether laparotomy renders the host more susceptible

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