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Merck
모든 사진(1)

주요 문서

L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

동의어(들):

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

UNSPSC 코드:
41106200
NACRES:
NA.85

Grade

Molecular Biology
for molecular biology

무균

sterile; 0.2 μm filtered

양식

liquid

기술

microbiological culture: suitable

pH

6.5-7.2

응용 분야

microbiology

저장 온도

room temp

적합성

nonselective for Escherichia coli
nonselective for coliforms

일반 설명

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

애플리케이션

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

특징 및 장점

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

성분

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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문서 라이브러리 방문

D O Chaffin et al.
Journal of bacteriology, 187(13), 4615-4626 (2005-06-22)
Several bacterial pathogens have evolved the means to escape immune detection by mimicking host cell surface carbohydrates that are crucial for self/non-self recognition. Sialic acid, a terminal residue on these carbohydrates, inhibits activation of the alternate pathway of complement by
Thomas P Burg et al.
Nature, 446(7139), 1066-1069 (2007-04-27)
Nanomechanical resonators enable the measurement of mass with extraordinary sensitivity. Previously, samples as light as 7 zeptograms (1 zg = 10(-21) g) have been weighed in vacuum, and proton-level resolution seems to be within reach. Resolving small mass changes requires
Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50
Antonio Basanta, Beatriz Gomez-Sala, et al.
Biotechnology, 76, 3314-3324 (2010)
Philippe M Fortune et al.
American journal of botany, 95(4), 454-464 (2008-04-01)
The origin of polyploid Bromus species of section Genea was investigated using molecular data. This group of annual species native from the Old-World is composed of three diploids, two tetraploids, one hexaploid, and one octoploid. Molecular cloning, sequencing, and phylogenetic
Felix Wezel et al.
International journal of molecular sciences, 22(6) (2021-04-04)
Invasive urothelial carcinomas of the bladder (UCB) characteristically show a loss of differentiation markers. The transcription factor Grainyhead-like 3 (GRHL3) plays an important role in the development and differentiation of normal urothelium. The contribution to UCB progression is still elusive.

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