L2650

Lectin from Glycine max (soybean)

peroxidase conjugate, lyophilized powder

• 동의어(들): SBA, Soybean agglutinin
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: Glycine max (soybean)
• 결합: peroxidase conjugate
• 분석: 85.00-100.00% (Warburg-Christian)
• 형태: lyophilized powder
• 효능: <80 μg per mL agglutination activity
• peroxidase activity: 25-100 units/mg protein
• 구성: Protein, ~95% modified Warburg-Christian
• 기술: western blot: suitable
• UniProt 수납 번호: P05046
• 저장 온도: −20°C
• 유전자 정보: soybean ... LE1(100818710)

설명

애플리케이션

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

생화학적/생리학적 작용

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

단위 정의

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

물리적 형태

Contains sodium citrate

제조 메모

Conjugates are prepared from affinity purified lectin.

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

분석 메모

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)