H5912

Anti-phospho-Histone H2AX (pSer¹³⁹) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 동의어(들): H2Ax Antibody, H2Ax Antibody - Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit, Anti-H2AXS139p
• MDL number: MFCD04974783
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen 15 kDa
• 종 반응성: mouse, human
• 기술: microarray: suitable; western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine
• UniProt 수납 번호: P16104
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: phosphorylation (pSer139)
• 유전자 정보: human ... H2AFX(3014) ; mouse ... H2afx(15270)

설명

일반 설명

H2AX comprises 2-25% of the total H2A complement in human cells, whereas in the budding yeast it constitutes virtually all of the H2A molecules. It has N-terminal and C-terminal region with a central globular domain. The C-terminal region has sites for modification including acetylation, biotinylation and phosphorylation.

면역원

The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX.

synthetic phosphorylated peptide corresponding to the C-terminus (amino acids 134-142) of human histone H2AX (pSer¹³⁹).

애플리케이션

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting.

생화학적/생리학적 작용

H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA. Ataxia-telangiectasia-mutated (ATM) is the major kinase involved in the phosphorylation of H2AX in response to DNA double-strand breaks. H2AX knockout mice are radiation sensitive and show retarted growth. They also are immune deficient with mutant males are infertile..

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable