추천 제품
생물학적 소스
mouse
Quality Level
결합
FITC conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
JBW301, monoclonal
종 반응성
human
제조업체/상표
Upstate®
기술
flow cytometry: suitable
immunocytochemistry: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
phosphorylation (pSer139)
유전자 정보
human ... H2AX(3014)
일반 설명
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
특이성
Broad species cross-reactivity is expected based on conservation of sequence homology.
Recognizes Histone H2A.X phosphorylated at Ser139, MW 15 kDa.
면역원
Epitope: Ser139
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse. Clone JBW301.
애플리케이션
Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton™ X-100 in TBS (5 min.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone.
품질
Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine.
Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.
Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.
표적 설명
15 kDa
물리적 형태
Protein G Purified
Purified FITC-conjugated mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide. Frozen solution.
저장 및 안정성
Stable for 1 year at from date of receipt.
분석 메모
Control
Staurosporine-treated Jurkat cells
Staurosporine-treated Jurkat cells
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Validation of a flow cytometry based G(2)M delay cell cycle assay for use in evaluating the pharmacodynamic response to Aurora A inhibition.
Estevam J, Danaee H, Liu R, Ecsedy J, Trepicchio WL, Wyant T
Journal of Immunological Methods null
Role of transcriptional corepressor CtBP1 in prostate cancer progression.
Wang, R; Asangani, IA; Chakravarthi, BV; Ateeq, B; Lonigro, RJ; Cao, Q; Mani, RS; Camacho et al.
Neoplasia null
David W Schoppy et al.
The Journal of clinical investigation, 122(1), 241-252 (2011-12-03)
Oncogenic Ras and p53 loss-of-function mutations are common in many advanced sporadic malignancies and together predict a limited responsiveness to conventional chemotherapy. Notably, studies in cultured cells have indicated that each of these genetic alterations creates a selective sensitivity to
Protective role of Puralpha to cisplatin.
Kaminski, R; Darbinyan, A; Merabova, N; Deshmane, SL; White, MK; Khalili, K
Cancer Biology & Therapy null
Gene deregulation and spatial genome reorganization near breakpoints prior to formation of translocations in anaplastic large cell lymphoma.
Mathas, S; Kreher, S; Meaburn, KJ; Johrens, K; Lamprecht, B; Assaf, C; Sterry, W; Kadin et al.
Proceedings of the National Academy of Sciences of the USA null
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.