추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, vertebrates
포장
antibody small pack of 25 μg
제조업체/상표
Upstate®
기술
ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
phosphorylation (pSer139)
유전자 정보
human ... HIST2H2AC(8338)
일반 설명
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
특이성
Broad species cross-reactivity expected based on conservation of sequence homology.
Histone H2A.X phosphorylated at Ser139.
면역원
Epitope: a.a. 134-142
애플리케이션
Anti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.
Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
품질
Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
표적 설명
15 kDa
결합
Replaces: MABE205
물리적 형태
Format: Purified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
분석 메모
Control
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Cells adapted to high NaCl have many DNA breaks and impaired DNA repair both in cell culture and in vivo.
Natalia I Dmitrieva, Qi Cai, Maurice B Burg
Proceedings of the National Academy of Sciences of the USA null
ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis.
Sanchez-Moran, E; Santos, JL; Jones, GH; Franklin, FC
Genes & Development null
High NaCl causes Mre11 to leave the nucleus, disrupting DNA damage signaling and repair.
Natalia I Dmitrieva, Dmitry V Bulavin, Maurice B Burg
American Journal of Physiology: Renal Physiology null
DNA replication is intrinsically hindered in terminally differentiated myotubes.
Pajalunga, D; Puggioni, EM; Mazzola, A; Leva, V; Montecucco, A; Crescenzi, M
Testing null
Absence of Wip1 partially rescues Atm deficiency phenotypes in mice.
Darlington, Y; Nguyen, TA; Moon, SH; Herron, A; Rao, P; Zhu, C; Lu, X; Donehower, LA
Oncogene null
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