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Merck
모든 사진(1)

주요 문서

H2508

Sigma-Aldrich

Hippuryl-Arg

carboxypeptidase substrate, chromogenic, ≥98% (TLC), powder

동의어(들):

N-Benzoyl-Gly-Arg

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About This Item

실험식(Hill 표기법):
C15H21N5O4
CAS Number:
Molecular Weight:
335.36
EC Number:
MDL number:
UNSPSC 코드:
12352204
PubChem Substance ID:
NACRES:
NA.32

제품명

Hippuryl-Arg, carboxypeptidase substrate

분석

≥98% (TLC)

양식

powder

solubility

water: 50 mg/mL, clear, colorless to faintly yellow

저장 온도

−20°C

SMILES string

NC(=N)NCCC[C@H](NC(=O)CNC(=O)c1ccccc1)C(O)=O

InChI

1S/C15H21N5O4/c16-15(17)18-8-4-7-11(14(23)24)20-12(21)9-19-13(22)10-5-2-1-3-6-10/h1-3,5-6,11H,4,7-9H2,(H,19,22)(H,20,21)(H,23,24)(H4,16,17,18)/t11-/m0/s1

InChI key

GFLCPYUSPYXNBV-NSHDSACASA-N

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관련 카테고리

애플리케이션

Hippuryl-Arg has been used as a substrate for:
  • aminopeptidase from Trogoderma granarium
  • exopeptidase from Bactrocera oleae Gmelin, midgut of Plagiodera versicolora
  • carboxypeptidase from Pichia pastoris

생화학적/생리학적 작용

Hippuryl-Arg is a synthetic substrate for carboxypeptidase B, and aminopeptidase. The enzyme carboxypeptidase acts on C terminal of arginine and lysine.

기질

Substrate for carboxypeptidase B and carboxypeptidase N.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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시험 성적서(COA)

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문서 라이브러리 방문

Hidefumi Komura et al.
Microbiology and immunology, 46(2), 115-117 (2002-04-10)
Carboxypeptidases (CP) in plasma and sera serve as regulators of anaphylatoxins such as C3a and C5a. The activity of CP can be measured by determining hippuric acid after cleavage of the small synthetic substrate hippuryl-L-arginine. Although a colorimetric assay is
Expression, purification and characterization of porcine pancreatic Carboxypeptidase B from Pichia pastoris for the conversion of recombinant human insulin
Mansur M, et al.
Enzyme and Microbial Technology, 40(3), 476-480 (2007)
Proteolytic activity in Plagiodera versicolora Laicharting (Coleoptera: Chrysomelidae): Characterization of digestive proteases and effect of host plants
Zibaee A and Hajizadeh J
Journal of Asia-Pacific Entomology, 16(3), 329-334 (2013)
H Schweisfurth et al.
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie, 21(10), 605-609 (1983-10-01)
Kininase I (carboxypeptidase N; EC 3.4.17.3) consists of carboxypeptidase N1 (CN1) and carboxypeptidase N2 (CN2); these two enzymes can be differentiated by their activities towards hippuryl-L-arginine and hippuryl-L-lysine, respectively. A spectrophotometric assay for both carboxypeptidases in human serum is described
Digestion and protein metabolism of Trogoderma granarium (Coleoptera: Dermestidae) fed on different barley varieties
Mardani-Talaee M, et al.
Journal of Stored Products Research, 73, 37-41 (2017)

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