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Key Documents

GE28-9893-36

Sigma-Aldrich

HiLoad® 26/600 Superdex® 200 pg

Cytiva 28-9893-36, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size

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About This Item

UNSPSC 코드:
41115700
NACRES:
SB.52

material

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

유통기한

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분자량

10000-600000 Da (globular proteins)

포장

pkg of 1 ea

제조업체/상표

Cytiva 28-9893-36

파라미터

<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa

베드 용적

320 mL

컬럼 길이 × I.D.

60 cm × 26 mm

기질

agarose-dextran composite
dextran-agarose particle platform
polymeric

기질 활성군

dextran-agarose phase
dextran-agarose phase

평균 부품 크기

34 μm

cleaning

1-14

working range

3-12

분리 기술

size exclusion (SEC)

관련 카테고리

일반 설명

HiLoad® 26/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice highly suitable for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

특징 및 장점

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

적합성

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

저장 및 안정성

Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

프로토콜

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

관련 콘텐츠

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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