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주요 문서

GE28-9893-35

Sigma-Aldrich

HiLoad® 16/600 Superdex® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

동의어(들):

16/600 Superdex 200, HiLoad Superdex 200

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About This Item

UNSPSC 코드:
41115700
NACRES:
SB.52

material

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

분자량

10000-600000 Da (globular proteins)

포장

pkg of 1 ea

제조업체/상표

Cytiva 28-9893-35

파라미터

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

베드 용적

120 mL

컬럼 길이 × I.D.

60 cm × 16 mm

Matrix

agarose dextran composite
dextran-agarose particle platform
polymeric

기질 활성군

dextran-agarose phase

평균 부품 크기

34 μm

cleaning

1-14

작동 범위

3-12

분리 기술

size exclusion (SEC)

일반 설명

HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

특징 및 장점

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

적합성

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


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시험 성적서(COA)

Lot/Batch Number

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관련 콘텐츠

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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