콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

GE28-9852-54

rProtein A GraviTrap

Cytiva 28-9852-54, pack of 10 × 1 mL

동의어(들):

GraviTrap

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

양식

prepacked column

교차

cross-linked

포장

pack of 10 × 1 mL

제조업체/상표

Cytiva 28-9852-54

저장 조건

(20% Ethanol)

기술

liquid chromatography (LC): suitable

베드 용적

1 mL

Matrix

(Highly cross-linked agarose, 4%)

기질 활성군

Protein A functional group

입자 크기

(90 µm)

용량

50 mg binding capacity (human IgG/column)(Protein binding capacity is protein-to-protein dependent.)

분리 기술

affinity

저장 온도

2-8°C

일반 설명

rProtein A GraviTrap includes ten ready-to-use columns prepacked with 1 mL of rProtein A Sepharose Fast Flow.

애플리케이션

rProtein A GraviTrap are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

특징 및 장점

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as rProtein A GraviTrap ~ 50 mg human IgG/column

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

프로토콜

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.