추천 제품
포장
pkg of 1 ea
제조업체/상표
Cytiva 28-9782-44
파라미터
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
베드 크기
7.7 mm × 100 mm
베드 용적
4.7 mL
컬럼 I.D.
7.7 mm
Matrix
6% cross-linked agarose
입자 크기
45-165 μm
평균 직경
90 μm
cleaning
2-14(Ni2+-stripped medium.)
작동 범위
3-12(Ni2+-stripped medium.)
용량
~40 mg binding capacity(histidine-tagged protein)
적합성
suitable for bioprocess medium
관련 카테고리
일반 설명
애플리케이션
HiScreen™ Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose™ 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.
특징 및 장점
- High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
- Prepacked, ready-to-use columns for convenient process development.
- Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
- Easy connection in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity
저장 및 안정성
분석 메모
법적 정보
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
문서
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
프로토콜
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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