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주요 문서

GE28-9782-44

HiScreen Ni Fast Flow

Cytiva 28-9782-44

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About This Item

UNSPSC 코드:
13111000
NACRES:
NA.56

포장

pkg of 1 ea

제조업체/상표

Cytiva 28-9782-44

파라미터

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

베드 크기

7.7 mm × 100 mm

베드 용적

4.7 mL

컬럼 I.D.

7.7 mm

Matrix

6% cross-linked agarose

입자 크기

45-165 μm

평균 직경

90 μm

cleaning

2-14(Ni2+-stripped medium.)

작동 범위

3-12(Ni2+-stripped medium.)

용량

~40 mg binding capacity(histidine-tagged protein)

적합성

suitable for bioprocess medium

관련 카테고리

일반 설명

HiScreen columns prepacked with Ni Sepharose 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

애플리케이션

HiScreen columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.

특징 및 장점

  • High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
  • Prepacked, ready-to-use columns for convenient process development.
  • Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
  • Easy connection in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

픽토그램

Environment

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


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시험 성적서(COA)

Lot/Batch Number

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도움이 필요하시면 연락하세요. 고객 지원 부서

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문서 라이브러리 방문

문서

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

프로토콜

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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