추천 제품
ligand
Cibacron Blue
포장
pkg of 1 ea
제조업체/상표
Cytiva 28-9782-43
저장 조건
(20% Ethanol, 0.1 M potassium phosphate)
(20% Ethanol, 0.9% NaCl, 1 mM Ca2₊ and 1 mM Mn2₊)
파라미터
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
베드 크기
7.7 mm × 100 mm
베드 용적
4.7 mL
컬럼 I.D.
7.7 mm
Matrix
6% cross-linked agarose
입자 크기
45-165 μm
평균 직경
90 μm
cleaning
3-13
작동 범위
4-12
적합성
suitable for bioprocess medium
저장 온도
2-8°C
일반 설명
HiScreen™ columns prepacked with Blue Sepharose™ Fast FLow are an excellent choice for method optimization and parameter screening for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.
애플리케이션
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
HiScreen™ Blue FF is prepacked with the BioProcess chromatography medium Blue Sepharose™ 6 Fast Flow. This medium has Cibacron Blue 3G covalently attached to the Sepharose™ 6 Fast FLow matrix by the triazine coupling method. The Blue dye binds many Proteins, such as albumin, interferon, lipoProteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).
HiScreen™ Blue FF is prepacked with the BioProcess chromatography medium Blue Sepharose™ 6 Fast Flow. This medium has Cibacron Blue 3G covalently attached to the Sepharose™ 6 Fast FLow matrix by the triazine coupling method. The Blue dye binds many Proteins, such as albumin, interferon, lipoProteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).
특징 및 장점
- Can be used for depletion of albumin.
- Prepacked, ready-to-use columns for convenient process development.
- Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
- Easy connection in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
프로토콜
This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from GE Healthcare.
Global Trade Item Number
SKU | GTIN |
---|---|
GE28-9782-43 | 4061837623769 |
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