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GE28-9537-66

HiTrap® TALON® Crude

Cytiva 28-9537-66, pack of 5 × 1 mL

동의어(들):

Crude TALON Column, HiTrap TALON, TALON Crude Column

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About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

포장

pack of 5 × 1 mL

제조업체/상표

Cytiva 28-9537-66

파라미터

1 ml/min - 4 ml/min flow rate
3 bar

컬럼 I.D.

7 mm

기질

6% cross-linked agarose

입자 크기

60-160 μm

cleaning

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

용량

≥20 mg binding capacity (histidine-tagged protein)

저장 온도

2-8°C

관련 카테고리

일반 설명

HiTrap® Talon® crude 1 mL and 5 mL columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

애플리케이션

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Talon® Superflow and prepacked HiTrap® Talon® crude columns allow Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

HiTrap® Talon® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the Talon® Superflow beads. Prior to loading samples on HiTrap® Talon® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.

특징 및 장점

  • Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
  • Prepacked HiTrap® Talon® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of Proteins.
  • HiTrap® Talon® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

HiTrap is a registered trademark of Cytiva
Talon is a registered trademark of Clontech

픽토그램

FlameFlame

신호어

Warning-Warning

유해 및 위험 성명서

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point (°F)

100.4 - 109.4 °F

Flash Point (°C)

38 - 43 °C


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

프로토콜

This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.

This page how to isolate proteins from inclusion bodies using GE Healthcare products.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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