GE28-9523-59

GST Spintrap^™

Cytiva 28-9523-59

• UNSPSC 코드: 41106500
• NACRES: NA.56

속성

• ligand: glutathione
• 포장: pkg of 50 ea
• 제조업체/상표: Cytiva 28-9523-59
• 베드 용적: 50 μL
• 기질: 4% agarose
• 입자 크기: 45-165 μm
• 평균 직경: 90 μm
• cleaning: 4-13
• working range: 4-13
• 용량: >25 mg binding capacity (horse liver GST/ml medium); ≥500 μg binding capacity(recombinant glutathione S-transferase (rGST))

설명

일반 설명

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.

특징 및 장점

• Preserves protein antigenicity and function.
• Yields up to 500 μg of pure protein per column.
• Allows direct purification of both clarified and unclarified cell lysates.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

SpinTrap is a trademark of Cytiva

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids