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Merck
모든 사진(1)

Key Documents

SAE0045

Sigma-Aldrich

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

동의어(들):

Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease

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About This Item

UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

human (human Rhinovirus Type 14)

Quality Level

분석

≥90% (SDS-PAGE)

형태

aqueous solution

특이 활성도

≥5000 U/mg

분자량

21 kDa

농도

0.8-1.2 mg/mL

기술

protein purification: suitable

적합성

suitable for protein modification

응용 분야

life science and biopharma

배송 상태

dry ice

저장 온도

−20°C

일반 설명

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

애플리케이션

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

단위 정의

One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

물리적 형태

Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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