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Merck
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주요 문서

GE28-9031-34

Protein G High Performance Spintrap

Cytiva 28-9031-34, pack of 16 columns

동의어(들):

Protein G Column

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.25

ligand

recombinant protein G lacking albumin-binding region

특징

wetted part: no

포장

pack of 16 columns

제조업체/상표

Cytiva 28-9031-34

저장 조건

(20% Ehtanol)
(20% Ethanol)

Matrix

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-9

작동 범위

3-9

용량

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(human IgG/column)

저장 온도

2-8°C

일반 설명

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein G HP SpinTrap is a prepacked, single-use spin column containing protein G Sepharose High Performance.

애플리케이션

Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).


There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

특징 및 장점

  • For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Prepacked with Protein G Sepharose High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein G HP prepacked columns.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

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문서 라이브러리 방문

문서

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

프로토콜

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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