GE28-4082-53

HiTrap^® MabSelect^™

Cytiva 28-4082-53, pack of 5 × 1 mL

• UNSPSC 코드: 23151816
• NACRES: NA.56

속성

• ligand: recombinant protein A (E. coli)
• 포장: pack of 5 × 1 mL
• 제조업체/상표: Cytiva 28-4082-53
• 저장 조건: (20% Ehtanol); (20% Ethanol)
• 파라미터: 42 psi
• 베드 크기: 7 mm × 25 mm
• 베드 용적: 1 mL
• 컬럼 I.D.: 7 mm
• Matrix: highly cross-linked agarose
• 평균 부품 크기: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-12
• 작동 범위: 3-10
• 용량: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• 적합성: suitable for bioprocess medium
• 저장 온도: 2-8°C

설명

일반 설명

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

애플리케이션

Lab Purification using the MabSelect^™ Protein A tag for capture of antibodies.

특징 및 장점

• HiTrap^® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

안전성 정보

• 픽토그램: GHS02
• 신호어: Warning
• 유해 및 위험 성명서: H226
• 예방조치 성명서: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids