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주요 문서

GE17-6002-33

HiTrap® IEX Selection Kit

Cytiva 17-6002-33

동의어(들):

IEX Selection Kit

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About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

ligand

quaternary amine

설명

Ion Exchanger Type (value)

포장

pkg of 7 ea

제조업체/상표

Cytiva 17-6002-33

파라미터

42 psi

베드 크기

7 mm × 25 mm

베드 용적

1 mL

컬럼 I.D.

7 mm

Matrix

6% cross-linked agarose

입자 크기

45-165 μm

평균 직경

90 μm

cleaning

2-14

작동 범위

2-12

적합성

suitable for bioprocess medium

관련 카테고리

일반 설명

HiTrap® IEX Selection Kit consists of seven HiTrap® columns, prepacked with different Sepharose Fast Flow ion exchange chromatography media for screening of the most appropriate media and conditions to use for specific application and development work.

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.

특징 및 장점

  • Fast and easy selection with seven different ion-exchange ligands on Sepharose Fast Flow and Sepharose XL, prepacked in 1 mL HiTrap® columns.
  • Offers a fast, easy and convenient way screen for the optimal media for a given application .
  • Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
  • Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP))
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

문서

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from GE Healthcare.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

모두 보기

프로토콜

This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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