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GE17-5268-01

Ni Sepharose™ High Performance

Cytiva 17-5268-01, pack of 25 mL

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

포장

pack of 25 mL

제조업체/상표

Cytiva 17-5268-01

Matrix

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning in place

2-14(Ni2+-stripped medium.)

작동 범위

3-12(Ni²⁺-stripped medium.)

용량

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

일반 설명

Ni Sepharose™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

특징 및 장점

  • High-performance purification of histidine-tagged proteins
  • Negligible leakage of Ni2+ ions.
  • High binding capacity, at least 40 mg/mL medium
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • 34 μm bead size for improved resolution

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

픽토그램

Environment
GHS09

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

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시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.

Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.

프로토콜

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from GE Healthcare.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using GE Healthcare products.

Handling Inclusion Bodies in Recombinant Protein Expression

This page how to isolate proteins from inclusion bodies using GE Healthcare products.

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