추천 제품
ligand
recombinant protein A (E. coli)
포장
pack of 25 mL
제조업체/상표
Cytiva 17-5199-01
기질
highly cross-linked agarose
평균 부품 크기
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning in place
2-12
working range
3-10
용량
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
적합성
suitable for bioprocess medium
저장 온도
2-8°C
일반 설명
애플리케이션
The medium is based on a highly cross-linked Agarose matrix with a recombinant Protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity medium with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool. MabSelect™ is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.
The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native Protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect™ an excellent choice for purification of MAbs at process scale.
MabSelect™ is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect™ meets industrial demands with security of supply and comprehensive technical and regulatory support.
특징 및 장점
- High-fLow Agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
- Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity which reduces media volume requirements
- Low non-specific binding resulting in Low levels of impurities in the product eluate pool
- BioProcess medium supported for industrial applications and well-established in approved processes.
저장 및 안정성
분석 메모
법적 정보
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
Capto adhere from GE Healthcare is a multimodal strong anion exchanger for BioProcess applications.
Description of viral clearance using Capto adhere from GE Healthcare.
This page describes the difference between bind/elute and flowthrough modes in multimodal chromatography using GE Healthcare products.
This page describes the optimization of loading conditions on Capto adhere from GE Healthcare using full factorial design of experiments (DoE).
프로토콜
This page shows how to pack Tricorn 10/100 columns with MabSelect or MabSelect SuRe from GE Healthcare.
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.